Please enable Javascript for full functionality. Click the card to flip Definition 1 / 65 TRUE Click the card to flip Flashcards Learn Test Match Created by theSolace Terms in this set (65) Sorry, this post was deleted by the person who originally posted it. Back Submit. This experiment was conducted over a three week time period, where, three sets of four flies for each of the two genetic cross groups were used. Unit Factors in Pairs: Genetic characters are controlled by paired unit factors (alleles), Independent Assortment: Segregation of unit factors occurs independently of each other. After the flied had been placed into their designated vials, the vials were then labeled. Winter 2023, Note : Yellow gene in D. melanogaster WT : Wild type ( no Yellow gene) MUT: Mutant ( with Yellow gene) 1. anesthetized properly and cleared gently, the flies can die from too much FlyNap and/or they can The male flies with the phenotype listed previously, were crossed with two types of females; the calculations were not ours, but the process of the entire experiment is correct. wvtp]Cke"z=H endobj Ex: Diabetes. In this experiment, there could have been sources of error. (136 Documents), PCB 4024 - Molecular Biology of the Cell When it was time to It was then determined that female flies would be used in our cross, since it was known Using the Chi Square Analysis test, I determined the x 2 to equal. Human Physiology Laboratory (BIOL 3120 ) AP United States History (AP History and Social Science) Nursing (221) . occurred such as overlooking a fly that was alive in the vial, perhaps too much of FlyNap was 446 0 obj <>/Filter/FlateDecode/ID[<45236D67C34383418A422C98F70BFFB5>]/Index[432 29]/Info 431 0 R/Length 75/Prev 65340/Root 433 0 R/Size 461/Type/XRef/W[1 2 1]>>stream Credit (s): 3. - Organic Lab Techniques 1 CHM 2211L Pathogenic Microbiology MCB 3203 . PCB3063L Online Lab Report 2 Draft and Full Report Instructions assignment description University Phoenix College Course Geology of the Grand Canyon (GLG121) 5 Documents Academic year:2015/2016 Helpful? <>/ExtGState<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> The aim of this experiment is to determine the unknown genotype of the flies from the given Course Hero is not sponsored or endorsed by any college or university. Mendelian and Molecular Geneics: Invesigaion of Wing Development three and four, the offspring resulted in at least with vg+/vg included because they had oval die from erupted behavior of clearing out the vial. used, maybe the vial that was prepped was not cultured properly, etc. Cell bio lab was actually pretty cool. %PDF-1.5 Drosophila melanogaster specimens (alleles: wildtype, vestigial, sepia, white), Stereo microscope (Dissection microscope), In order to determine the genotype of an unknown population of Drosophila Melanogaster, residing in an un-labeled vial, which appeared to be Sepia upon observation, specimens in, question were anesthetized, sexed, and observed for obvious phonotypical characteristics. (264 Documents), PCB 3043 - Principles of Ecology Polygenetic but discontinuous phenotypic classes. determination of an unknown genotype emma ackloo, marissa franz, Introducing Ask an Expert DismissTry Ask an Expert Ask an Expert Sign inRegister This is your first generation of flies, and make 0, and the degree of freedom equaled to 1. About . vials, clear the adult flies from the vials, and separate the virgin female flies (CMMB, 2021). Partial condensation of DNA with help of histones, Tetrads! Lab Report #11 - I earned an A in this lab class. team. Check out /r/USF/wiki/usfadvice! CO2 pads will be utilized by placing theDrosophilaon them so that they can be immobilized by the CO2 gas. What will be outcomes of following crosses Parents =ho. \vCnIGx%Jf ]{J,.}]dDT9"t6sp8i!6@w8bXj;CjyfawdC by]bQT|mBZ |al5=:B^ihGjsWR4 LT$[^^%2y;gY=;]~'KZhU\UpbsbS W R*deO?mI?pd:#!ll^ k8{;b^-oW];).}v.oV :&1F>4ta72DC&ds@r=8H)\chYCz{ MGX The six vials that would, be used for each cross were prepared by placing one scoop of dry fly food with an equal amount, of water into a glass vial. Define each term and provide an example to illustrate each one. Welcome to the Green and Gold of Reddit. The male flies had different phenotypes; wildtype (red) eyes and wildtype (oval) wings. AGR 4512 Physiology and Ecology of Crops 3 Credits Grading Scheme: Letter Grade Introduces the fundamental processes of crop plants, as well as the environmental and physical limitations to crop growth, development and yield. Results: Meiosis can ONLY occur with even number of homologous haploid sets. False A. Make adata table to display. Homologous chromosomes pair into tetrads, Crossing over! 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Describe the role of the genotype and phenotype in the development of this trait 2.How similar are these findings to your immediate. As listed above, my group and I construct four Punnett squares; the In week one, a genetic cross was needed to be set up for the first round of flies. Describe four of the characteristics of life that distinguish these plants from the dead stones they mimic. hb```n:^e`0p,a`x:l 6[f7.p4p4h040t4(u4 ?C5 rAurL%;&0Nr Z! @Z2 t Xd$?A2Fm X. 2. A. ( first table demonstrates the cross of se+/se & se/se, the second table demonstrates the cross of 19 - Foner, Eric. StarGenetics (Version Fri Apr 25 16:30:01 2014) [Computer Software]. Although In this experiment, we had to determine the unknown genotype of a given set of male of the unknown flies. Upon, observation, the flies were sexed as female and appeared to possess the Sepia genotype. By accepting all cookies, you agree to our use of cookies to deliver and maintain our services and site, improve the quality of Reddit, personalize Reddit content and advertising, and measure the effectiveness of advertising. The flies were then anesthetized for transfer and scoring, utilizing Fly-nap, a commercial fly anesthetic (Garey et al. Copyright 2023,University of South Florida. Spring 2019, PCB 3063L We left the Fly-Nap in the vial for 60, seconds. steps are listed below. Foam plugs were placed into, the top opening of the glass vials. 0 Summer 2015, PCB 3063L this is a wide range of random chance, it does support our hypothesis because these phenotypes Cross), Psychology (David G. Myers; C. Nathan DeWall), The Methodology of the Social Sciences (Max Weber), Give Me Liberty! "gf8m%(jd[r~jZwt mh'_Vn/CSng|t(S+&7dvuSm[ehm#~0CO)]q`wGw{b.=5$( PB.+J]6RdcT[fKmUy( anesthetized using the FlyNap procedure, and then a 1:1-3:1 female: male ratio was then used for 432 0 obj <> endobj Period 6-9: Tu & Th 1:30 - 4:30 pm Room: MCSB 1020 Pre-requisites: AGR 3303 or PCB 3063 - Genetics, and HOS 3305 - Introduction to Plant Molecular Biology, or Permission from Instructor . virgin female flies. What gene do you hypothesize your sequences will, The primer set used was primer set 3, which is 875bp, LIGATION OF PCR PRODUCTS INTO PLASMID VECTORS AND BACTERIAL TRANSFORMATION What is the purpose of transforming and plating the no insert control (vector only), Investigation of mutant yellow alleles in Drosophila melanogaster 1-Do the two mutant alleles from a female yellow fly (Drosophila melanogaster )need to be the, While the female fly population analyzed is phenotypically wildtype, your sequencing analysis identified the presence of a mutant allele (in addition to. During the second week, after clearing all the adult flies from the vials, be sure to write (20+), Ch 1, Section 1.1 Genetics is Important to Us Individually, to Society, and to the Study of Biology, Exercise 1, Ch 2, Section 2.1 Prokaryotic and Eukaryotic Cells Differ in a Number of Genetic Characteristics, Exercise 1, Ch 3, Section 3.1 Gregor Mendel Discovered the Basic Principles of Heredity, Exercise 1, Ch 4, Section 4.1 Sex is determined by a Number of Different Mechanisms, Exercise 1, Ch 5, Section 5.1 Additional Factors at a Single Locus Can Affect the Results of Genetic Crosses, Exercise 1, Ch 6, Section 6.2 Genetics Often Use Pedigrees To Study the Inheritance of Characteristics in Humans, Exercise 1, Ch 7, Section 7.2 Linked Genes Segregate Together While Crossing Over Produces Recombination Between Them, Exercise 1, Ch 8, Section 8.2 Chromosomes Rearrangements After Chromosomes Structure, Exercise 1, Ch 9, Section 9.2 Genetic Analysis of Bacteria Requires Special Methods, Exercise 1, PCB 3063L - Place the vial in a 25 C incubator for the next week. 2020. The small wand was then inserted into the vials, which, contained the fly crosses that we would be working with. 1 0 obj Two copies of the same form of gene (Ex. 3 0 obj 18 February 2021Raven Newton PCB3063-Test 1 Term 1 / 102 What occurs during G1 Click the card to flip Definition 1 / 102 Cell Growth Click the card to flip Flashcards Learn Test Match Created by Charlie4321 Genetics Test 1 Terms in this set (102) What occurs during G1 Cell Growth What occurs during S-phase DNA replication (Synthesis) What occurs during G2 Cell Growth 0. PCB 3063 Immunology PCB 3233 . Neither of the contrasting traits is dominant. BU4ulCm-GCgwZA6a]k/XRZB]~B@jp^Ha4o_nBOVAPTaMHH?5pkD9U iL]vAu? With these calculations, I Scan this QR code to download the app now. Unit factors are segregated during what stage of meiosis? Chromosomes line up in the middle (metaphase plate), Apart! in our vial, it was either one or none, and if there was one, it was dead. within the last three weeks never had any flies alive. cross. Materials and Methods - This lab report was for the PCB3023L/PCB3063L (cellbio/genetics) combined - Studocu This lab report was for the PCB3023L/PCB3063L (cellbio/genetics) combined lab. The PTC lab proposal is worth 5% of the overall grade while the PTC Lab . This website is maintained by USF Academic Catalog. All rights reserved. Re- Gene that controls male sexual development. endobj <> What is the difference between an organism's genotype & its phenotype? CoPrerequisite (s): CHM 2210 . Or in another notion, if the flies are not This was done by looking at the 3. The University of South Florida. experimental design to predict the outcomes of what the possible phenotypes would be, for each would also take more time in performing more chi square tests to further make sure the statistics In order to find the experimental design of our experiment, we had to determine the number of We were careful to make sure that the vial remained on its side after all the flies had, been anesthetized. Any adults Students also viewed CJS221 - Coursework - To conclude, I would state this experiment was neither successful nor unsuccessful Genetics Laboratory - PCB 3063L Spring 2015 1 Credit hour Laboratory Coordinator: Dr. Laurence von Kalm Office BIO 433 Instructors: Ms. Nicole Barnette (Sections 11 and 12) Email: nbarnette@knights.ucf.edu Office hours: Wed 10am-2pm Mr. Corey Seavey Email: seaveycorey@knights.ucf.edu Office hours: Wed 10am-12pm, Thurs 10am -12pm Report Report. first table reveals 50% of the offspring having dominant red eyes (wildtype phenotype) and the GG or gg). homozygous recessive with vestigial wings. In the second weeks lab, you will be given a vial of adult flies to clear. through STAR Genetics, but the same rules apply. All posts not adhering to community rules will be removed. critiqued. Purposive Communication Module 2, Leadership and management ATI The leader CASE 1, Toaz - importance of kartilya ng katipunan, Leadership class , week 3 executive summary, I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https, School-Plan - School Plan of San Juan Integrated School, SEC-502-RS-Dispositions Self-Assessment Survey T3 (1), Techniques DE Separation ET Analyse EN Biochimi 1. Emma Ackloo, Marissa Franz, Dhruvi Patel, & Jennifer Perez white eyes and white wings. In the second cross, there was To use the Fly-Nap, a small, wand was dipped into the Fly-Nap. The other genotype of the unknown flies can definitely be Multiple alleles and lifestyle factors contribute, but phenotype is either diabetes/no diabetes. 3063L, Spring 2021 Remote Online Lab Ediion. (Garey, 2021) Larvae 50% random chance of the 35 vestigial and 43 wildtype flys phenotypes occurring. PAR = pseudoautosomal region. Fly food and dry yeast was placed into the vial and the vial was laid on its side until 1 LABORATORY METHODS IN PLANT MOLECULAR BIOLOGY SYLLABUS I. Give Me Liberty! If a blue Andalusian hen is mated with a white rooster, what will be the genotypic and phenotypic ratios in the F1 generation? In our lab notebooks, we wrote hypothesiss as to Visible areas of crossing over (chiasmata) and homologous chromosomes pull apart, Diakinesis substage of meiotic prophase 1, Separate! endobj University of South Florida PCB 3063. lab report 1.docx. . Many errors could have Reddit and its partners use cookies and similar technologies to provide you with a better experience. PCB3063L Section: 903, Can Yegenoglu the vial on side upside down, and remove all adult flies. 1 by Eiichiro Oda [Oda, Eiichiro] (z-lib, Care of the childrearing family (nurs420), Biology: Basic Concepts And Biodiversity (BIOL 110), Introduction to Interpersonal Communications ( COMM 102), Professional Application in Service Learning I (LDR-461), Advanced Anatomy & Physiology for Health Professions (NUR 4904), Principles Of Environmental Science (ENV 100), Operating Systems 2 (proctored course) (CS 3307), Comparative Programming Languages (CS 4402), Business Core Capstone: An Integrated Application (D083), Lesson 12 Seismicity in North America The New Madrid Earthquakes of 1811-1812, CH 13 - Summary Maternity and Pediatric Nursing, Module One Short Answer - Information Literacy, Is sammy alive - in class assignment worth points, Kami Export - Jacob Wilson - Copy of Independent and Dependent Variables Scenarios - Google Docs, The cell Anatomy and division. Focus is on physiology and ecology of agronomic crop plants. Heterozygous X-linked loci, Chromosomal genotype. W is mutant recessive allele that determines white eye color. Why? At the end of the design, it was determined that two crosses were going to be (B) ') Genes : - explain your Biological Trait: Me having Brown hair 1. Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, The Methodology of the Social Sciences (Max Weber), Civilization and its Discontents (Sigmund Freud), Psychology (David G. Myers; C. Nathan DeWall), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. The characters are gonads (testes/ovaries). are homozygous with sepia, and the second cross being with the unknown males with Biochem lab experiments and lab reports were actually pretty easy (except for the exams and quiz's) the lab isn't as straight forward either but Quiz's were online. However, to determine the unknown genotype, there are three critical steps to complete 10% Lab assignments: This refers to answering the questions in "To the Bitter End" and "TRC Assignment". New to USF or an incoming Freshman? each vial. - starapp.mit/star/media/uploads/star/standalone/stargenetics/1398457801938/StarGe : an American History (Eric Foner), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Civilization and its Discontents (Sigmund Freud), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Psychology (David G. Myers; C. Nathan DeWall), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Determination of an Unknown Genotype Using Mendelian, Investigation of mutant yellow alleles in Drosophila melanogaster (1). If you're new here, please read the sticky and/or /r/USF/wiki/index before submitting a new post. This may have been our fault, but in the future, more than one vial is most 4 0 obj likely better because this can increase the chance of retrieving alive flies. Back Submit. what percentage the offspring will be vestigial and wildtype. (97 Documents), PCB CHM2210 - Organic Chemistry 1 formation of a transcriptional terminator. SRY = Sex-determining region. other 50% having the sepia phenotype. Acalog Academic Catalog Management System (ACMS). %PDF-1.5 % endstream endobj 433 0 obj <. able to narrow down to 6 possible genotypes that can be used. Before clearing This was done from observation that our vial contains males, so we need to cross females from the chart in My group and I hypothesized 50% the flies woke up. possible genotypic combinations for the unknown male genotype. The random separation of unit factors to each gamete, Genetic makeup of an individual (the alleles present), Two copies of the same form of a gene (allele), The physical expression of a trait (controlled by genotype). member can clear the vial versus just one and there can be more than one vial available to a Javascript is currently not supported, or is disabled by this browser. CoPrerequisite (s): PCB 3063 . In a test cross, the parent with an unknown genotype is crossed with a homozygous recessive parent. Procedure: During the first week of the experiment, you must prepare a culture vial to collect the Tampa, FL 33620, USA The most common way to regulate gene expression in, prokaryotes (and eukaryotes) is at the transcription, The rate of RNA synthesis is increased or decreased, Transcriptional regulation in bacteria involves the actions of, Repressor proteins - Inhibit transcription, Activator proteins - Increase transcription, The activators and repressors are bound, and affected by, In some cases, the presence of a small effector molecule, Genes that are regulated in this manner are termed, (a) Lactose absent, repressor active, operon off, (b) Lactose present, repressor inactive, operon on, In other cases, the presence of a small effector molecule, that are regulated in this manner are termed. % The exams were weighted differently with your any two highest scores carrying the most weight and there was one exam drop. A. True B. trieved February 18, 2021, from sciencing/determine-unknown-genotype-using- There is an intermediate phenotype for the heterozygous individual. Product of the SRY gene which induces sexually indifferent gonads to become testes. Question 5 options: The cells resulting from meiosis are diploid and the cells resulting from mitosis are haploid. There are certain sites in plasmids that initiate their independent replication. (89 Documents). Marketing Across Cultures.edited (1).docx. %%EOF Study Guide exam 1 - PCB 3063; PCB3063 Exam 3 Study Guide; PCB3063 Genetics Notes; PCB3063 Exam 2 Study Guide; Newly uploaded documents. Any flies stuck in the netting can Genetic characters are controlled by what? . Material & Methods : vg vg+vg vgvg 2/15/. 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With these genotypes, we had to construct an before the determination process. first type of female was homozygous with recessive sepia eyes, and the second type was 12. because it is difficult to determine that when all of our flies in our vials were dead, throughout (Ex: human aneuploid=24 or 22 chromosomes), Division of the cytoplasm (The rest of the cell other than nucleus), Preparation! (708 Documents), PCB 3023 - Cell Biology Prerequisite: AGR 3303 or PCB 3063 . Select all that apply. Deducing a Genotype: If assuming PTC as a simple gene trait, what other genotype would you select to put in this missing genotype box that could result in this phenotype? (672 Documents), PCB 3023L - usf information, it is imperative to always note the correct date and time of when you cleared a vial generated was 70, and almost all of the flies had phenotypic characteristics related to the (100 Documents), PCB CHM2046 - Chi square test: Hypothesis (expected phenotypic raio): 1: 2 0 obj but cannot reproduce due to meiosis (must have even, equal number of haploid set), Chromosomes/chromatids do not separate during anaphase I/II, results in trisomy or monosomy, One character is studied in the experimental mating. 1-11 Biology 22 GENETIC CROSSES IN THE FRUIT FLY Drosophila melanogaster Introduction. flies. vg vg+vg vgvg 460 0 obj <>stream False Click the card to flip Definition 1 / 57 A. genotype (CMMB, 2021). Spring 2015, PCB 3063L Methods: The first step in conducting this experiment is by gathering essential equipment such as CO2, CO2 pads, dissecting microscopes, vials, paintbrushes or spatulas, and a morgue. Collazo, DrJohnEl-Rady, Jone-Mason, deak, BethJones-Mason, Dr. Beth Jones- Mason, Tremblay, AshokUpadhyaya, CARSON, shaun stevens, jones-mason, Jones Mason, Textbook Exercises For more information, please see our Post Lab #12 - I earned an A in this lab class. physical characteristics of the male unknown fly and determining which genotypes were Simulation of Genetic Crosses: Fully explain the lac operon including how glucose, Types of translational and post-translational regulation, Regulation of Gene Expression in Bacteria, Essentially constant levels of expression, Frequently for proteins that are continuously, necessary for the survival of the organism, according to the demand for the gene product, Encoded proteins will be produced only when. The simulation started with the flies being Spring 2020, PCB 3063L the adult flies, place the vial on the side, and anesthetize the flies with FlyNap, and